VINIF.2021.DA00163 – Mushroom of biodiversity hotspots in Vietnam: edible records, yields, seasons, and cultivation possibilities

Principle Investigator
Dr. Pham Nguyen Duc Hoang
Host Organization
Institute of Mycology and Biotechnology, Vietnam Union of Science and Technology Associations

Establish a database of large mushroom species in different forest systems in Vietnam to record the potential to be used as food, estimate production, crop season and screen the cultivability of the edible forest mushrooms which appear in large quantities.

Main tasks of the project

  • Survey, set up sample plots and sampling routes at: 4 sample plots, each sample plot is 4 hectares (200m x 200m), set up 3 sampling lines with perimeter of 3 concentric squares within an area of 4 hectares, 1 hectare and 0.25 hectare respectively. Sample plots are established at Tam Dao National Park, Thuong Tien Nature Reserve, Da Nhim Protection Forest and Dong Nai Cultural Nature Reserve.
  • Collect mushroom samples along sampling lines and sample plots continuously for 1 year: Collect samples once every 2 weeks, 23 times/year on all 4 sample plots (Do not collect samples during the dry and rainy seasons in the north). Collect fungal samples, describe morphology in the field, collect molecular biology samples, record ecological data such as sample weight, temperature, air humidity and rain/sunshine at the sampling areas. Dry, number and preserve samples. Isolate fungal samples on agar medium.
  • Identification of mushroom samples: DNA extraction and preliminary identification based on ITS sequences of all samples, comparison with morphological description to identify detailed samples in the group of edible/poisonous mushrooms that have been recorded in the world.
  • Storing, preserving, and investigating the mycelial development ability of isolated edible mushroom varieties.
  • Screen and select 1-2 potential edible mushroom species and experimentally cultivate them.
  • Process data, write reports and publish results.

Project impact

  • Forest mushrooms are a valuable resource that can be used both as food and as traditional medicine. Understanding this resource helps us manage and exploit it effectively.
  • While natural forest mushrooms are sold at high prices in the market, every year there are still many cases of death due to eating poisonous mushrooms. Descriptive data on edible and poisonous mushrooms can help local communities make better use of natural resources, especially non-timber forest products, in planting and protecting forests. In addition, molecular biological data on poisonous mushrooms helps poison control centers develop rapid test kits to detect poisonous agents for better treatment regimens.
  • Data on yield and harvest timing provide local communities with better harvesting strategies to increase income while keeping resources sustainable.
  • New species and new records from the region are announced and added to the list of major mushrooms in Vietnam.
  • The collection of dried specimens helps future studies to test, compare, and revise the project’s research results as well as supplement more accurate and scientific data on Vietnamese large mushrooms.
Principle Investigator
Dr. Pham Nguyen Duc Hoang
Host Organization
Institute of Mycology and Biotechnology, Vietnam Union of Science and Technology Associations

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Expect Progress
15/11/2021
15/08/2022
Phase 1

– Collection of dried specimens: Minimum of 200 dried specimens with complete initial descriptions and molecular biology data.
– Support for undergraduate and postgraduate training: Recognition of 02 Research Fellows and supervisors with dissertation titles related to the project content.

15/08/2023
Phase 2

– Collection of dried specimens: Minimum of 500 well-preserved dried specimens under optimal conditions. All specimens should have complete data regarding their initial descriptions and molecular biology.
– Collection of germplasm: Minimum of 20 strains preserved on slanted agar in test tubes. These strains should have comprehensive databases regarding classification, viability, and cultivation characteristics.
– Publication in Q1-ranked international journals or equivalent: Manuscript draft has been confirmed for English language proofreading (Professional Editing).
– Support for undergraduate and postgraduate training: Recognition of 01 decision to admit a postgraduate student.

15/11/2024
Phase 3

Publication in Q1-ranked international journals or equivalent: Letter of acceptance for publication or already published.
Reference book: Completion of a 100-150 page manuscript (with a publishing decision). Database of edible and poisonous mushroom species in natural forests in Vietnam. The book contains scientific content, is valuable for reference and teaching purposes, and is reliable for citation in other studies.
Useful solution patent: Decision accepting a valid patent application. Related to the cultivation/use of mushroom species obtained from the project.
Support for undergraduate and postgraduate training: Certificate of completion of Master’s degree with a thesis related to the project content.

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