VINIF.2023.DA156 – Phage therapy – a potential solution in the fight against antimicrobial resistance (AMR) in aquaculture in Vietnam: a study from phage bank, -omics analysis, to farm trial in striped catfish fingerling production

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Principle Investigator
Assoc.Prof. Hoang Anh Hoang
Host Organization
Ho Chi Minh City University of Technology, Vietnam National University - Ho Chi Minh City

Project goals

Successful in establishing a phage bank: isolation and assessment of bacterial and phage activity; Set up records, processes, introduce and advertise phage banking.

Successful in -omics analysis: genome, immune response, fish intestinal microbiota, effects of phages on the diversity of water and pond mud bacteria.

Successful in researching and evaluating the effectiveness of phage preparations against hemorrhagic and purulent liver and kidney diseases in striped catfish seeds at pond scale.

Main content of the project

Establishing a phage bank: isolation and assessment of bacterial and phage activity; Set up records, processes, introduce and advertise phage banking.

–omics analysis: genome, immune response, fish intestinal microbiota, effects of phages on the diversity of water and pond mud bacteria.

Researching and evaluating the effectiveness of phage preparations against hemorrhagic and purulent liver and kidney diseases in striped catfish seeds at pond scale.

Project impact

The project contributes to expanding research on bacteriophages in Vietnam. The first phage bank will be established, contributing to research and technology transfer in Vietnam. The project is also a good opportunity to strengthen cooperation with research groups in Vietnam and abroad.

Enhance technology transfer and strengthen the value chain of the striped catfish industry in Vietnam.

The project contributes to adjusting policies on sustainable development and environmental protection in general and in aquaculture in particular in Vietnam.

project manager image
Principle Investigator
Assoc.Prof. Hoang Anh Hoang
Host Organization
Ho Chi Minh City University of Technology, Vietnam National University - Ho Chi Minh City

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Expect Progress
01/11/2023
01/11/2024
Phase 1

1. Collect samples for isolation, screening, purification, and identification of A. hydrophila and E. ictaluri bacteria from catfish ponds.
2. Evaluate the toxicity of the isolated bacteria on a fish model.
3. Collect samples for the isolation of probiotic bacteria strains.
4. Participate in national and international scientific conferences in the field.

01/11/2025
Phase 2

1. Isolate probiotic bacterial strains.
2. Evaluate the characteristics of the probiotic bacterial strains.
3. Collect samples for isolation, screening, purification, and identification of A. hydrophila and E. ictaluri bacteria from catfish ponds.
4. Assess the toxicity of the isolated bacteria using a fish model.
5. Sequence and analyze the genomes of the probiotic bacterial strains.
6. Analyze the impact of probiotic bacterial strains on the gut microbiota of fish.
7. Analyze the impact of probiotic bacterial strains on the immune system of fish.
8. Establish a probiotic bacterial strain bank.
9. Participate in national and international scientific conferences.
10. Prepare scientific paper submissions.
Conduct training sessions.

01/11/2026
Phase 3

1. Evaluate the effectiveness of probiotic strain incorporation into fish feed pellets.
2. Produce probiotic formulations.
3. Test probiotic formulations on a pond scale.
4. Assess the impact of probiotic strains on the aquatic microbial community in pond water and sediment.
5. Develop strategies for using probiotic strains to control key bacterial diseases in commercial-scale catfish fingerling production.
6. Patent applications.
7. Prepare scientific paper submissions.
8. Participate in national and international scientific conferences.
9. Conduct training sessions.

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