VINIF.2020.NCUD.DA240 – AgriBiosol – A safe, organic and sustainable agro-biotechnology solution

Principle Investigator
Mr. Nguyen Van Minh, Ms. Duong Nhat Linh
Host Organization
MIDOLI Co., Ltd

The project includes 2 solutions and includes two sets of biological products. The success of the project helps complete 12 products, through testing will complete a number of effective usage processes for conversion, organic farming and shrimp farming, aquaculture without using antibiotics:

  1. MICROSOL – Microbiological technology solution to replace antibiotics in shrimp farming. This is a proven 100% antibiotic replacement solution that still helps shrimp reach the finish line even in epidemic areas. The solution provides a set of microbiological products to effectively control pathogenic bacteria, especially Vibrio parahaemolyticus carries the toxin gene that causes acute hepatopancreatic necrosis and provides 2 procedures for use in shrimp ponds to help farmers optimize activity and save money. The solution also provides stable effects on water environmental indicators such as toxic gases: NH3, NO2, H2S and supports digestion. This solution was also honored to be awarded the first prize in the Innovation contest in high-tech agricultural production jointly organized by the High-Tech Agricultural Business Incubation Center and the Department of Science and Technology of Ho Chi Minh City.
  2. AGRIBIS – Biotechnology solution to transform agricultural farming towards organic, safe and sustainable. The solution provides a set of microbiological and herbal products that help effectively control pests, leafhoppers, aphids, bacteria, fungi, root nematodes, red spiders,… and stimulate plant growth. The solution also provides processes for use from the beginning of the season, from composting, soil preparation, etc. to reduce up to 100% replacement of chemical pesticides. The solution also provides an effective process of converting chemically cultivated land to a safe and organic one thanks to the microorganism system that decomposes chemical pesticides and provides selected strains of soil improvement microorganisms. This solution is also honored to receive funding from the SpeedUp Foundation – the Innovation and Startup Support Program for the period 2016 – 2020 of the Department of Science and Technology of Ho Chi Minh City.

The product set is designed from a collection of indigenous microorganisms (endogenous in plants and rhizosphere) capable of producing many biological compounds that come with a specific scientific background with many actives proven to be beneficial for shrimp and plants from in vitro to in vivo in combination with herbal ingredients extracted from plants. Each product has its own mixing formula with high density production and spore generation technology to help increase survivability, maintain stable activity and preserve for a long time. The product is in powder or liquid form and is being researched into a compact tablet form, convenient for use and transportation. The model is convenient for project replication and proactive implementation on farms. In addition, we research plasma seed treatment technology to increase germination rate, resist fungal diseases, create disease-free seeds, and ensure crop quality right from the first stage of the process, contributes to increasing productivity. Bacteriophage technology is also used to optimize antibacterial ability, has high specificity, so the dosage is low, the protection period is long, stable in the environment, kills bacteria quickly, effective for a long time, and does not harm humans, plants, or pets. The project has the potential to register exclusive patents/useful solutions and is all based on the accumulated results of more than 15 years of experience from 32 scientific works and awards related to the project that have been published, verified and practically tested on many commercial shrimp ponds and crop farms such as Sadec Farm organic tangerine garden, Lam Vien – Duc Trong – Lam Dong organic vegetable garden, organic pepper garden in MangYang – Gia Lai, organic red flesh dragon fruit garden in Chi Cong commune – Tuy Phong – Binh Thuan,…

Principle Investigator
Mr. Nguyen Van Minh, Ms. Duong Nhat Linh
Host Organization
MIDOLI Co., Ltd

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Expect Progress
15/05/2020
14/11/2020
Phase 1 (First 06 months)

Product:

– Spore creation technology of 2 Bacillus strains achieving high density ≥ 1010 CFU/mL(g) (high density bacillus strains will help optimize costs and increase preservation time of products using 2 strains this Bacillus);

– Fermentation technology, obtaining crude enzymes: amylase, cellulase, protease (these enzymes will be mixed into 2 aquatic products: MicroSol AQRich-S: supports digestion for shrimp, prevents liver necrosis disease pancreas, white feces disease for shrimp and MicroSol AQRich-F: supports digestion for fish, prevents purulent liver and kidney disease in fish);

– Formulated to increase the ability to prevent two-spotted red spiders (Tetranychus urticae) (this technology is to optimize the insecticidal activity of BIOMI-Pest 1 (tablet) – CPVS insecticide and BIOMI-Pest 2 (liquid) – Pesticide control method);

– Technological process to optimize the fermentation environment of 2 bacterial strains to improve activity (this technology is to optimize the resistance activity for BIOMI-AntiFB 1 (tablet);

-CPVS prevents fungi, bacteria and BIOMI-AntiN 1 (tablets);

– CPVS for biological control of root-damaging nematodes);

– Formula to increase the ability to kill gray caterpillars (Agrotis ipsilon) (this technology is to optimize the insecticidal activity for BIOMI-Pest 1 (tablet) – insecticide CPVS and BIOMI-Pest 2 (liquid) – Pesticide CPVS);

– Formula combining microbial strains that promote strong activity in 4 aquatic products (Microsol AClean-S, MicroSol AQRich-S, Microsol AClean-F, MicroSol AQRich-F);

– The tablet production process ensures the density of microbial strains of 4 aquatic products (Microsol AClean-S, MicroSol AQRich-S, Microsol AClean-F, MicroSol AQRich-F) and 04 complete biological products. in tablet form (Microsol AClean-S, MicroSol AQRich-S, Microsol AClean-F, MicroSol AQRich-F)

– 1st 6-month report.

14/05/2021
Phase 1 (Next 06 months)

Product:

– Technology to extract insecticidal Bt toxin (this technology aims to optimize insecticidal activity for BIOMI-Pest 1 (tablet) – insecticide CPVS and BIOMI-Pest 2 (liquid) – insecticide CPVS);

– Report and recommend appropriate cold plasma treatment time to increase germination rate and fungal disease resistance (part of the product “Process of converting farming to safe, organic, sustainable agriculture” sustainable to meet the effectiveness of pathogen control, productivity and environmental protection”);

– Production process and product of liquid microbial organic fertilizer (BIOMI- Ferti (liquid): Microbial organic fertilizer stimulates plant growth);

– Production process of 1 liquid insecticide (BIOMI-Pest 2 (liquid): insecticide);

– Results of microbial density of products after 6 months (4 products Microsol AClean-S, MicroSol AQRich-S, Microsol AClean-F, MicroSol AQRich-F)

– Second 6-month report.

14/11/2021
Phase 2 (First 06 months)

Product:

– Microbiological product set to control pathogens, treat water, and support digestion for shrimp in commercial ponds; Technological process uses a set of microbial products to replace 100% of antibiotics in shrimp farming;

– Microbiological product set to help stimulate growth, prevent and resist fungi, pests and diseases in tangerine gardens (Tri-BIOMI 3X, BIOMI- NitroFix, BIOMI-AntiFB 1, BIOMI-AntiN 1, BIOMI-Pest 1, BIOMI -Pest 2 , BIOMI- Ferti); The process of converting farming to safe, organic, sustainable agriculture meets the effectiveness of pathogen control, productivity and environmental protection;

– Microbiological product set to help stimulate growth, prevent and resist fungi, pests and diseases in pepper gardens (Tri-BIOMI 3X, BIOMI- NitroFix, BIOMI-AntiFB 1, BIOMI-AntiN 1, BIOMI-Pest 1, BIOMI-Pest 2, BIOMI-Ferti); The process of converting farming to safe, organic, sustainable agriculture meets the effectiveness of pathogen control, productivity and environmental protection;

– Microbiological product set to help stimulate growth, prevent and resist fungi, pests and diseases in durian gardens (Tri-BIOMI 3X, BIOMI- NitroFix, BIOMI-AntiFB 1, BIOMI-AntiN 1, BIOMI-Pest 1, BIOMI-Pest 2, BIOMI-Ferti); The process of converting farming to safe, organic and sustainable agriculture meets the effectiveness of pathogen control, productivity and environmental protection.

– Microbiological product set to help stimulate growth, prevent and resist fungi, pests and diseases on rice (Tri-BIOMI 3X, BIOMI- NitroFix, BIOMI-AntiFB 1, BIOMI-AntiN 1, BIOMI-Pest 1, BIOMI- Pest 2 , BIOMI- Ferti); The process of converting farming to safe, organic, sustainable agriculture meets the effectiveness of pathogen control, productivity and environmental protection;

– Information license for circulation of 4 microbial products used in aquaculture (Microsol AClean-S, MicroSol AQRich-S, Microsol AClean-F, MicroSol AQRich-F) – Nitrogen-fixing microbial fertilizer in tablet form assay (BIOMI- NitroFix)”;

– Results of microbial density of products after 12 months (4 products Microsol AClean-S, MicroSol AQRich-S, Microsol AClean-F, MicroSol AQRich-F);

– Whole genome sequence data set of 5 bacterial strains;

– Third 6-month report

15/05/2022
Phase 2 (Next 06 months)

Product:

– There are about 1-2 bacteriophage strains with inhibitory activity against V.parahaemolyticus (raw material for MicroSol Phage-S product);

– Bacteriophage preparations in the seafood sector as sample products (MicroSol Phage-S: bacteriophage that kills bacterial pathogens);

– 03 accepted applications – Marketing Plan;

– Organize scientific conferences:

+ Microbiological technology solutions to replace antibiotics in shrimp farming

+ Biotechnology solutions for safe, organic and sustainable agricultural farming;

– E-commerce website;

– Final Report.

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